Watching 'jumping genes' in action: Real-time observation of transposon activity in living cells
6/13/2016 1:19 PM
Reporting in the Proceedings of the National Academy of Sciences, scientists at the University of Illinois at Urbana-Champaign have observed jumping gene activity in real time within living cells. The study is the collaborative effort of physics professors Thomas Kuhlman and Nigel Goldenfeld, at the Center for the Physics of Living Cells, a National Science Foundation Physics Frontiers Center.
"In this study, we were able to see that there is actually more of this jumping gene action going on than might have been expected from previous studies,” said Kuhlman, whose team performed the in vivo experiments. “What’s more, we learned that the rates at which these genes jump depend sensitively on how the cells are growing—if there is food available for the cells to grow, for example. In other words, jumping gene activation isn’t entirely random, it’s dependent on environmental feedback."
To observe these individual cellular-evolution events in living cells, Kuhlman’s team devised a synthetic biological system using the bacterium Escherichia coli. The scientists coupled the expression of fluorescent reporters—genes that encode (in this case, blue and yellow) fluorescent proteins—to the jumping activity of the transposons. The scientists could then visually record the transposon activity using fluorescent microscopy.
In order to help design the experiment and to calculate what would have happened if the jumping occurred in a purely random fashion, Goldenfeld’s team developed computer simulations of the growth of bacterial colonies and predicted what the experimental signal would look like in the random case. These calculations showed that the experiments could not simply be interpreted as random transposon activity and even provided clues as to sources of non-randomness, including environmental feedback and heredity.
“Our work involved a good deal of computational image analysis, followed by statistical analysis,” said Goldenfeld. “To extract signals and conclusions from the raw data, simulation and theoretical calculations were integral to the experimental design and interpretation. This sort of collaborative project was really only possible with the unique structure provided by the Center for the Physics of Living Cells.”
E. coli cells carrying a “wildtype” version of the transposable element grow in a monolayer within a microfluidic device; a single cell is seen to light up green upon experiencing a transposable element event. Video courtesy of T.E. Kuhlman, University of Illinois at Urbana-Champaign
“The over-arching long-term research goal is a deeper understanding of how evolution works at the molecular level. Direct observation of how genomes in cells restructure themselves allows for a precise determination of adaptation rates and may shed light on a host of important evolutionary questions, ranging from the emergence of life to the spread of cancer, where cells undergo rapid mutations and transformations of their genomes,” added Kuhlman.
Kuhlman and Goldenfeld are members of the Carl R. Woese Institute for Genomic Biology and the Department of Physics at the University of Illinois at Urbana-Champaign. The work involved several graduate students, including lead author Hyuneil Kim, Gloria Lee, Nicholas Sherer and Michael Martini.
This release is based upon research performed at the Center for the Physics of Living Cells, a Physics Frontiers Center supported by the National Science Foundation, and at the NASA Astrobiology Institute for Universal Biology supported by NASA, both located on the University of Illinois at Urbana-Champaign campus. Kuhlman’s research is additionally supported by a 2015 Sloan Research Fellowship from the Alfred P. Sloan Foundation. The conclusions presented are those of the scientists, and not necessarily those of the funding agencies.