CPLC/iPoLS Seminar: "MINFLUX Nanoscopy and Related Matters"
(sign-up)Stefan Hell, Max Planck Institute
|Event Contact:||Sharlene Denos
|Sponsor:||UIUC Center for the Physics of Living Cells(CPLC)/International Physics of Living Systems(iPoLS)|
I will show how an in-depth description of the basic principles of diffraction-unlimited fluorescence microscopy (nanoscopy) [1-3] has spawned a new powerful superresolution concept, namely MINFLUX nanoscopy . MINFLUX utilizes a local excitation intensity minimum (of a doughnut or a standing wave) that is targeted like a probe in order to localize the fluorescent molecule to be registered. In combination with single-molecule switching for sequential registration, MINFLUX [4-6] has obtained the ultimate (super)resolution: the size of a molecule. MINFLUX nanoscopy, providing 1–3 nanometer resolution in fixed and living cells, is presently being established for routine fluorescence imaging at the highest, moleculars ize resolution levels. Relying on fewer detected photons than popular camera-based localization, MINFLUX nanoscopy is poised to open a new chapter in the imaging of protein complexes and distributions in fixed and living cells.
Stefan Hell is a director at both the Max Planck Institute for Biophysical Chemistry in Göttingen and the Max Planck Institute for Medical Research in Heidelberg, Germany.
Photo: © Peter Badge/Typos1 in cop. with Foundation Lindau Nobel Laureate Meetings - all rights reserved 2017.
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Webinar ID: 857 3839 8354
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