Collaborative research team solves cancer cell mutation mystery

Susan McKenna, Alex Kreig, and Siv Schwink
5/18/2015

Research breakthrough has implications for better targeted cancer treatment protocols

 

Professor of Physics and of Bioengineering Jun Song
Professor of Physics and of Bioengineering Jun Song
More than 500,000 people in the United States die each year of cancer-related causes. Now, emerging research has identified the mechanism behind one of the most common mutations that helps cancer cells to replicate limitlessly.

Approximately 85 percent of cancer cells obtain their limitless replicative potential through the reactivation of a specific protein called telomerase (TERT). Recent cancer research has shown that highly recurrent mutations in the promoter of the TERT gene are the most common genetic mutations in many cancers, including adult glioblastoma and hepatocellular carcinoma.

TERT stabilizes chromosomes by elongating the protective element at the end of each chromosome in a cell. Scientists have discovered that cells harboring these mutations aberrantly increase TERT expression, effectively making them immortal.

Now, a collaborative team of researchers at the University of Illinois at Urbana-Champaign and at the University of California, San Francisco, has uncovered the mechanisms by which mutations result in elevated TERT expression. The team’s findings, published in the May 14 issue of Science, have exciting implications for new, more precise and personalized cancer treatments with fewer side effects compared with current treatments.

Physics Illinois postdoctoral researcher Hans Tomas Rube played a key role in the discovery
Physics Illinois postdoctoral researcher Hans Tomas Rube played a key role in the discovery
By integrating computational and experimental analyses, the researchers, led by Illinois biological physicist Jun S. Song and UCSF cancer biologist Joseph Costello, identified that the mechanism of increased TERT expression in tumor tissue relies on a specific transcription factor that selectively binds the mutated sequences. A transcription factor is a protein that binds specific DNA sequences and regulates how its target genes are expressed (in this case the gene that expresses TERT). Thus, the TERT mutations act as a new binding site for the transcription factor that controls TERT expression. The newly identified transcription factor does not recognize the normal TERT promoter sequence, and thus, does not regulate TERT in healthy tissue.

The team’s work further showed that the same transcription factor recognizes and binds the mutant TERT promoter in tumor cells from four different cancer types, underscoring that this is a common mechanism of TERT reactivation.

The identified transcription factor and its regulators have great potential for the development of new precision therapeutic interventions in cancers that harbor the TERT mutations. A treatment that would inhibit TERT in a targeted cancer-cell-specific manner would bypass the toxicities associated with current treatments that inadvertently also target TERT in normal healthy cells.

Based on these new findings, the team is now conducting a variety of experiments designed to test whether inhibiting the transcription factor activity would not only turn down TERT expression, but might also result in selective cancer cell death.

This project was enhanced by the complementary analysis conducted by three research groups located across the country. Joseph F. Costello’s laboratory at UCSF is linked to the UCSF Medical Center and the Helen Diller Family Comprehensive Cancer Center, which allowed for access to human tumor samples that generated the cell cultures and produced relevant models. Jun Song's group at Illinois provided advanced computational analysis of the genomic data and predictions that narrowed in on possible mechanisms behind the previously identified mutation. Finally, through single-molecule analysis, Su-A Myong’s lab at Illinois provided verification that the proposed mechanism operated in the suggested matter.

The researchers at Illinois include H. Tomas Rube, Alex Kreig, Sua Myong, and Song; the UCSF collaborators include Robert J. A. Bell, Andrew Mancini, Shaun F. Fouse, Raman P. Nagarajan, Serah Choi, Chibo Hong, Daniel He, Melike Pekmezci, John K. Wiencke, Margaret R. Wrensch, Susan M. Chang, Kyle M. Walsh, and Joseph F. Costello.

This research was funded by by NCI R01CA163336 (J.S.S.), the Sontag Foundation Distinguished Scientist Award (J.S.S.), NCI P50CA097257 and P01CA118816-06 (M.S.B., S.M.C., J.F.C.); NCI R01CA169316-01 (J.F.C.), the Grove Foundation, the Karen Osney Brownstein Endowed Chair (J.F.C.), the Anne and Jason Farber Foundation, and a gift from the Dabbiere family. Additional support was provided by NCI R25CA112355 (K.M.W.), R01CA52689 (K.M.W., J.K.W., M.R.W.), P50CA097257 (K.M.W., M.R.W.), the Stanley D. Lewis and Virginia S. Lewis Endowed Chair in Brain Tumor Research, the Robert Magnin Newman Endowed Chair in Neuro-oncology, the Founder Professorship from the Grainger Engineering Breakthroughs Initiative (J.S.S.), and donations from families and friends of John Berardi, Helen Glaser, Elvera Olsen, Raymond E. Cooper, and William Martinusen. Provisional patent application (application number: 62/145,579) has been filed by the University of California, San Francisco, and University of Illinois. All transcriptome sequencing data have been deposited in the European Genome-phenome Archive under accession number EGAS00001001242.

Recent News

  • Research

In a paper published today in Physical Review Letters, a team of researchers led by Illinois Physics Professor Nicolás Yunes present two conclusions. First, using NICER’s observation datamand universal relations among various properties of neutron stars, the authors infer the moment of inertia; the tidal Love number; the quadrupole moment; and the surface eccentricity of neutron star PSR J0030+045. Next, they use their inferences to propose and implement a novel test of GR.

  • Outreach

Illinois Physics Professor Smitha Vishveshwara has been elected to the Executive Committee of the American Physical Society (APS) Division of Condensed Matter Physics (DCMP). By special election, Vishveshwara fills the seat of Richard L. Greene of University of Maryland, who stepped down from the position of chair-elect.

The DCMP chair line typically represents a four-year commitment of service, and each newly elected member generally starts as vice chair for the first year, then serves as chair-elect, chair, and past chair. Vishveshwara will serve as chair-elect starting immediately, and will take over as chair in March 2022.

A new textbook from Cambridge University Press entitled Numerical Relativity: Starting from Scratch, coauthored by Bowdoin College Physics Professor Thomas W. Baumgarte and Illinois Physics and Astronomy Professor Stuart L. Shapiro, explicates this esoteric subfield of physics for today’s students and scientists. The textbook makes heavy use of analogies from Newtonian gravity, scalar fields, and electromagnetic fields. In this way, it introduces key concepts of numerical relativity in a context familiar to readers without prior expertise in general relativity. Readers can explore the concepts presented by working through textbook exercises, and can see them first-hand by experimenting with the accompanying Python sample codes.

  • Accolades

Three University of Illinois Urbana-Champaign professors have been elected to the National Academy of Sciences, one of the highest professional honors a scientist can receive.

Physics professor Nadya Mason and chemistry professors Ralph Nuzzo and Wilfred van der Donk are among 120 newly elected U.S. members – 59 of whom are women, the most elected in a single year – and 30 international members in recognition of their distinguished and continuing achievements in original research.