Professor Evilevitch received his PhD in Physical Chemistry at Lund University, Sweden in 2001. For two years, he was a STINT postdoctoral fellow at University of California, Los Angeles, where he began his research in virus biophysics. He became an Assistant Professor and later an Associate Professor at the Department of Chemistry at Lund University, Sweden. From 2009-2016 he held an Associate Professor appointment in the Department of Physics at Carnegie Mellon University inÂ Pittsburgh, before he joined University of Illinois at Urbana-Champaign.
- Evilevitch laboratory in Virus Biophysics focuses on key physical mechanisms for infectivity and replication of double-stranded DNA viruses. Experimental model systems used in the group are dsDNA bacteriophages and human herpes viruses (e.g. HSV-1). We are specifically interested in physical mechanisms of DNA packaging and ejection from viral capsids, viral packaging motors, DNA structural transitions inside the capsids associated with infection, viral DNA ejection dynamics, assembly and mechanical stability of viral capsids, effects of molecular crowding on viral DNA ejection and viral replication in vivo, viral DNA condensation. The current research direction is centered around group’s discovery of high internal DNA pressure inside human Herpesvirus capsids. This DNA pressure reaches tens of atmospheres! The internal genome pressure is generated by strong repulsive interactions between tightly packaged, negatively charged DNA strands as well as DNA bending energy. This mechanical DNA pressure is responsible for initiation of viral infection. Also central to our laboratory is the direct link between virus biophysics and molecular genetics. The ability to selectively modify viral genes of interest allows identification of specific protein domains required for DNA encapsidation and retention during capsid assembly and viral capsid maturation. We have the unique capability to perform both single molecule and bulk measurements on viruses under controlled solution conditions. The main techniques are atomic force microscopy, ultra-sensitive microcalorimetry, fluorescence microscopy, light scattering, solution X-ray (SAXS) and neutron scattering (SANS).
Evilevitch laboratory conducts research in virus biophysics. Specifically, we investigate how physical forces control viral infectious cycles. Since physical forces are often more universal than specific biochemical molecular interactions, this knowledge helps us to understand not just how one type of virus works, but how many classes of viruses replicate, providing insight into virus evolution and how to interfere with viral infections in a novel "biophysical" way. Our main tools are solution X-ray and neutron scattering, atomic force microscopy, ultra-sensitive microcalorimetry and fluorescence microscopy.
More on the Lab website: http://vetmed.illinois.edu/evilevitch-lab/
- 2008 Sven och Ebba-Christina Hagberg's Prize in Biochemistry and Medicine from the Royal Swedish Academy of Sciences and Karolinska University, Stockholm, Sweden
- 2005 Akzo Nobel Nordic Science Prize
- 2004 Swedish Research Council Career Award
- 2004 The UCLA Chancellor's Award for Postdoctoral Research with Exceptional Accomplishments in research
- 2003 The 2003 Hebert Newby McCoy Award, UCLA
- 2002 Award for Postdoctoral Research from STINT (The Swedish Foundation of International Cooperation in Research and Higher Education)
Selected Articles in Journals
- K. Freeman, M.A. Behrens, K.A. Streletzky, U. Olsson, A. Evilevitch. "Portal stability controls dynamics of DNA ejection from Phage," J Phys Chem 2016 May 13. doi: 10.1021/acs.jpcb.6b04172.
- D.W. Bauer, A. Evilevitch. "Influence of Internal DNA Pressure on Stability and Infectivity of Phage lambda," J Mol Biol. 2015 Aug 5. doi: 10.1016/j.jmb.2015.07.023. [Epub ahead of print]
- D.W. Bauer, D. Li, J. Huffman, F.L. Homa, K. Wilson, J.C. Leavitt, S.R. Casjens, J. Baines, A. Evilevitch. "Exploring the balance between DNA pressure and capsid stability in Herpes and phage," J Virol. 2015 Jul 1. pii: JVI.01172-15. [Epub ahead of print]
- D. Li, T. Liu, X. Zuo, T. Li, X. Qiu, A. Evilevitch. "Ionic switch controls the DNA state in phage lambda," Nucleic Acids Research 2015, June 19. pii: gku634. [Epub ahead of print]
- U. Sae-Ueng, T. Liu, C. E. Catalano, J. B. Huffman, F. L. Homa, A. Evilevitch. "Major capsid reinforcement by a minor protein in herpesviruses and phage," Nucleic Acids Research 2014, Jul 22. pii: gku634. [Epub ahead of print]
- U. Sae-Ueng, D. Li, X. Zuo, J.B. Huffman, F.L. Homa, A. Evilevitch. "Solid-to-fluid DNA transition inside HSV-1 capsid close to the temperature of infection," Nature Chemical Biology, 2014. 07 September 2014; doi:10.1038/nchembio.1628.
- T. Liu, U. Sae-Ueng, D. Li, G. Lander, X. Zuo, B. Jönsson, D. Rau, I. Shefer, A. Evilevitch. "Solid-to-fluid-like DNA transition in viruses facilitates infection," PNAS 2014; published ahead of print September 30, 2014, doi: 10.1073/pnas.1321637111
- David Bauer, Jamie Huffman, Fred Homa, Alex Evilevitch. "Herpes Virus Genome, The Pressure is On," Journal of the American Chemical Society, July 5, 2013, 135 (30), pp 11216-11221; doi: 10.1021/ja404008r
- A. Evilevitch. "Physical evolution of pressure-driven viral infection," New and Notable Article, Biophysical Journal, Volume 104, Issue 10, 2113-2114, 21 May 2013; doi 10.1016/j.bpj.2013.03.062.
- G. C. Lander, J. E. Johnson, D. C. Rau, C. S. Potter, B. Carragher, A. Evilevitch. “DNA bending induced phase transition of encapsidated genome in phage lambda,” Nucleic Acids Research, 28 February 2013; doi 10.1093/nar/gkt137.
- Aylin Ahadi, Dan Johansson, A. Evilevitch. “Modeling and simulation of the mechanical response from nanoindentation test of DNA-filled viral capsids,” Journal of Biological Physics, March 2013; doi 10.1007/s10867-013-9297-9.
- Emrecan Soylemez, Maarten P. de Boer, Udom Sae-Ueng, Alex Evilevitch, Tom A. Stewart, May Nyman. "Photocatalytic Degradation of Bacteriophages Evidenced by Atomic Force Microscopy," PLoS One, January 3, 2013, Vol. 8, Issue 1, e53601.
- Elmar Nurmemmedov, Martin Castelnovo, Elisabeth Medina, Carlos E Catalano, Alex Evilevitch. "Challenging packaging limits and infectivity of phage lambda," Journal of Molecular Biology, 13 January 2012, Vol. 415, Issue 2, pp 263-273.
- Pernilla Nevsten, Alex Evilevitch, and Reine Wallenberg. “Chemical mapping of DNA and counter-ion content inside phage by energy-filtered TEM,” Journal of Biological Physics, March 2012, Vol. 38, Issue 2, pp 229-240.
- A. Evilevitch, W. H. Roos, I. L. Ivanvoska, M. Jeembaeva, B. Jönsson, and G. J. L. Wuite. “Effects of Salts on Internal DNA Pressure and Mechnical Properties of Phage Capsids,” Journal of Molecular Biology, Vol. 405, Issue 1, 2011.
- M. Jeembaeva, B. Jönsson, M. Castelnovo, and A. Evilevitch. “DNA Heats Up: Energetics of Genome Ejection from Phage Revealed by Isothermal Titration Calorimetry,” Journal of Molecular Biology, Vol. 395, Issue 5, 2010.
- S. Koester, A. Evilevitch, M. Jeembaeva, and D. Weitz. “Dependence of Capsid-Pressure Dependence on Viral Infection by Phage Lambda,” Biophysical Journal, Vol. 97, Issue 6, 2009.
- A. Ahadi, J. Colomo, A. Evilevitch. “Simulation of Nanoindentation Response of Viral Capsids. Shape and Size Effects,” J. of Phys. Chem. B, Mar 19; 113 (11):3370-8, 2009.
- G. Lander, A. Evilevitch, M. Jeembaeva, C. S. Potter, B. Carragher, J. E. Johnson. “Bacteriophage Lambda Stabilization by Auxiliary Protein gpD: Timing, Location, and Mechanism of Attachment Determined by Cryo-EM,” Structure, 16: 1399-1406, 2008.
- M. Jeembaeva, M. Castelnovo, F. Larsson, A. Evilevitch. "Osmotic pressure: inhibiting or promoting DNA ejection from phage," J. Mol. Biol. Aug 29; 381(2):310-23, 2008. Epub 2008 June 6.
- A. Evilevitch, L. Fang, A. Yoffe, M. Castelnovo, D. C. Rau, V. A. Parsegian, W. M. Gelbart, C. M. Knobler. “Effects of salt concentrations and bending energy on the extent of ejection of phage genomes,” Biophysical Journal, Feb, Vol. 94 1110-1120, 2008.
- E. Nurmemmedov, M. Castelnovo, C.E. Catalano, A. Evilevitch. "Biophysics of viral infectivity: matching genome length with capsid size." Q Rev Biophys. 2007 Nov; 40 (4):327-56. Review.
- M. Castelnovo and A. Evilevitch. “DNA ejection from bacteriophage: towards a general behavior for osmotic suppression experiments,” Eur. Phys. J. E 24, 9-18, 2007.
- D. Löf, K. Schillén, B. Jönsson, A. Evilevitch. "Dynamic and static light scattering of DNA ejection kinetics from phage," Physical Review E, Vol. 76, No 1, 011914, 2007.
- W. Roos, I. Ivanovska, A. Evilevitch, G. Wuite. “Viral capsids: mechanical characteristics, genome packaging and delivery mechanisms,” review article, Cellular and Molecular Life Sciences Journal, 64 (12): 1484–1497, June 2007.
- I. Ivanovska, B. Jönsson, G. Wuite, A. Evilevitch. “Internal DNA pressure modifies stability of wild-type phage,” Proceedings of the National Academy of Sciences of the United States of America (PNAS), Jun 5; 104 (23):9603-8. Epub 2007 May 29, 2007.
- D. Löf, K. Schillén, B. Jönsson, A. Evilevitch. “Forces controlling the rate of DNA ejection from phage lambda.” Journal of Molecular Biology, Apr 20; 368 (1):55-65. Epub 2007 Feb 6, 2007.
- A. Evilevitch, “Effects of condensing agent and nuclease on the extent of ejection from phage lambda,” Journal of Physical Chemistry B, 110, 22261-22265, 2006.
- M. Castelnovo, A. Evilevitch. “Binding Effects in Multivalent Gibbs-Donnan Equilibrium,” Europhys. Lett., 4, 73, 2006.
- P. Grayson, A. Evilevitch, M.M. Inamdar, P.K. Purohit, W.M. Gelbart, C.M. Knobler, R. Phillips. "The effect of genome length on ejection forces in bacteriophage lambda," Virology. 2006 May 10; 348 (2):430-6. Epub 2006 Feb 15.
- A. Evilevitch, J.W. Gober, M. Phillips, C.M. Knobler, W.M. Gelbart. “Measurements of DNA lengths remaining in a viral capsid after osmotically suppressed partial ejection,” Biophysical Journal 88 (1): 751-756 Jan 2005.
- A. Evilevitch, M. Castelnovo, C.M. Knobler, W.M. Gelbart. “Measuring the force ejecting DNA from phage,” Journal of Physical Chemistry B 108 (21): 6838-6843 May 27 2004.
- A. Evilevitch, L. Lavelle, E. Raspaud, C.M. Knobler, W.M. Gelbart. “Osmotic pressure inhibition of DNA ejection from phage,” Proceedings of the National Academy of Sciences of the United States of America (PNAS) 100 (16): 9292-9295 Aug 5 2003.
- A. Evilevitch, J. Rescic, B. Jönsson, U. Olsson. “Computer simulation of molecular exchange in colloidal systems,” Journal of Physical Chemistry B 106 (45): 11746-11757 Nov 14 2002.
- A. Evilevitch, B. Jönsson, U. Olsson, H. Wennerström. “Molecular transport in a nonequilibrium droplet microemulsion system,” Langmuir 17 (22): 6893-6904 Oct 30 2001.
- A. Evilevitch, V. Lobaskin, P. Linse, U. Olsson. “Structure and transport properties of a charged spherical microemulsion system,” Langmuir 17 (4): 1043-1053 Feb 20 2001.
- A. Evilevitch, U. Olsson, B. Jönsson, H. Wennerström “Kinetics of oil solubilization in microemulsion droplets. Mechanism of oil transport,” Langmuir 16 (23): 8755-8762 Nov 14 2000.