Ido Golding

Professor

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Ido Golding

Primary Research Area

  • Biological Physics
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Biography

Professor Ido Golding received his Ph.D in physics from Tel Aviv University (Israel) in 2001. Originally trained as a condensed matter theorist, he later spent five years learning the experimental arsenal of modern molecular biology.

From 2002 to 2006, Professor Golding was a Lewis Thomas Research Fellow in the Department of Molecular Biology at Princeton University. He joined the faculty of the Department of Physics at the University of Illinois at Urbana-Champaign in 2007. In 2019, Professor Golding returned to the Department of Physics from Baylor College of Medicine, where he was a Professor of Biochemistry and Molecular Biology.

Professor Golding is a member of the Center for the Physics of Living Cells and the Center for Theoretical Biological Physics.

 

Research Statement

In our lab, we examine the way living cells process information from their environment and make decisions based on that information.

Our aim is to form a quantitative narrative for the dynamics of cellular decision-making and unveil simple principles that underlie such processes. Our first model system is the bacterium E. coli and its virus, phage lambda. We examine their complex interaction at the level of individual events in space and time. More recently, we have begun to extend our studies to higher organisms.

Work in the lab involves a set of skills broader than what is generally mastered within a single discipline, including the techniques of molecular- and cell biology; live single-cell microscopy for making quantitative measurements; and data analysis using the engineer's toolbox of signal- and image processing; all accompanied by the theoretical tools of dynamical systems theory, stochastic processes, non-equilibrium phenomena and more.

The practice of modern in vivo biology, combined with the intellectual effort of a quantitative approach, will contribute significantly to a young scientist's training experience, better preparing them for the future world of "Systems Biology".

Prospective students and post-docs of all backgrounds (physics, biology, chemistry, engineering, etc.) are welcome to contact me.

Semesters Ranked Excellent Teacher by Students

SemesterCourseOutstanding
Fall 2008PHYS 102

Selected Articles in Journals

  • I. Golding, Single-cell studies of phage lambda: Hidden treasures under Occam's rug, Annual Reviews of Virology 3 (1) (2016).
  • L.A. Sepulveda, H. Xu, J. Zhang, M. Wan and I. Golding, Measurement of gene regulation in individual cells reveals rapid switching between promoter states, Science 351 (6278), 1218-1222 (2016).
  • H. Xu, S. O. Skinner, A. M. Sokac and I. Golding, Stochastic Kinetics of Nascent RNA, Physical Review Letters 117 (12) 128101 (2016).
  • A. Sanchez and I. Golding, Genetic determinants and cellular constraints in noisy gene expression, Science 342 (6163), 1188-1193 (2013).
  • I. Golding, Decision making in living cells: Lessons from a simple system, Annual Review of Biophysics 40:63 80 (2011).
  • L. Zeng, S. O. Skinner, C. Zong, J. Sippy, M. Feiss and I. Golding, Decision making at a subcellular level determines the outcome of bacteriophage infection, Cell 141 (4) 682-691 (2010).
  • L. H. So, A. Ghosh, C. Zong, L. A. Sepulveda, R. Segev and I. Golding, General properties of the transcriptional time series in Escherichia coli, Nature Genetics 43:554 560 (2011).
  • I. Golding, E.C. Cox. Physical nature of bacterial cytoplasm. Phys. Rev. Lett. 96, 098102 (2006).
  • I. Golding, J. Paulsson, S.M. Zawilski, E.C. Cox. Real-time kinetics of gene activity in individual bacteria. Cell 123 (6) 1025-1036 (2005).

Related news

  • Faculty Highlights
  • Biological Physics
  • Biophysics

As a biological physicist, Ido Golding studies the function of living cells. He is best known for the experimental quantification of key biological processes, such as gene expression and viral infection, inside individual bacterial cells.

  • Research
  • Biological Physics
  • Biophysics

Scientists studying genetic transcription are gaining new insights into a process that is fundamental to all life. Transcription is the first step in gene expression, the process taking place within all living cells by which the DNA sequence of a gene is copied into RNA, which in turn (most generally speaking) serves as the template for assembling protein molecules, the basic building blocks of life.

Much of what scientists have uncovered about transcription over the past five decades is based on bulk investigative techniques employing large numbers of living cells. Today, advanced imaging techniques allow scientists to probe the inner workings of transcription at the scale of individual genes, and a new more detailed picture of this vital process is emerging.

Just this week, two new in vivo single-molecule studies of transcription in E. coli were published by scientists at the University of Illinois at Urbana-Champaign, one by Professor Ido Golding and colleagues, unveiling unexpected and up-to-now hidden drivers of cellular individuality; the other by Professor Sangjin Kim and colleagues, demonstrating for the first time that transcription dynamics are affected by molecular-scale long-distance communication between RNA polymerase (RNAP) molecules while they are “reading” a gene sequence one base at a time and assembling the complementary RNA strand.